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Mycology

Training Manual - Mycology (177 Pages)

Lesson 1 - Introduction to Medical Mycology

History
Classification of Fungi
Cultivation
Macroscopic Appearance of Fungi
Microscopic Structures
Mycoses
Exercises
Solutions


Lesson 2 - Collection and Processing Procedures for Mycological Studies

Abscess Specimens
Biopsy Material
Blood Cultures
Bone Marrow
General Spinal Fluid
Hair, Skin and Nails (Dermatophytes)
Draining Fistula, Sinus Tract
Joint Fluid
Nasopharyngeal
Pleural Fluid
Sputum, Bronchial Washings
Thrush Lesions of Oral Cavity
Urine Specimen
Exercises
Solutions


Lesson 3 - Saprophytic Fungi

Section I. Introduction
Reasons for Studying
Potential Clinical Importance
Opportunistic Infections

Section II. Zygomycetes
Mucor Species
Rhizopus Species
Syncephalastruh Species
Absidia Species

Section III. Deuteromycetes
Introduction to Deuteromycetes (Fungi Imperfecti)
Aspergillosis
Alternaria
Cladosporium
Drechslera
Curvularia
Penicillium
Paecilohyces
Nigrospora
Scopulariopsis
Trichoderma
Fusarium
Sepedoniuh
Exercises
Solutions


Lesson 4 - Yeasts of Medical Importance

Candidiasis
Candida Albicans
Candida Tropicalis
Differentiation of Candida Species
Geotrichum Candiduh
Cryptococcus
Rhodotorula
Torulopsis
Saccharomyces
Exercises
Solutions


Lesson 5 - Cutaneous Fungi

Section I. Superficial Fungi
Malassezia Furfur
Exophiala Herneckii
Piedraia Hortae
Trichosporon Bbigblli

Section II. Dermatophytes
Microsporum SPP
Epidermophyton Floccosuh
Trichophyton
Diseases Caused by Dermatophytes
Exercises
Solutions


Lesson 6 - Subcutaneous Fungi

Mycetoma
Chromomycosis
Rhinosporidiosis
Sporothrichosis
Exercises
Solutions


Lesson 7 - Systemic Mycoses

Histoplasmosis
Blastomycosis
Paracoccidioidomycosis
Coccidioidomycosis
Exercises
Solutions


Lesson 8 - Mycological Procedures

Section I. Specimen Processing
Potassium Hydroxide with Glycerol
India Ink Reagents and Test
Lactophenol Cotton Blue Stain
Slide Culture

Section II. Organism Identification
Germ Tube Test
Sterile Rice Grain Test
Urease Test
In Vitro Hair Test
Periodic Acid-Schiff Stain
Saline Wet Mount
Cut-Streak Method for Morphology of Yeasts
Exercises
Solutions


Lesson 9 - Media and Reagents

Section I. Media
Isolation Media
Sabouraud's Dextrose Agar
Sabouraud Dextrose Agar with Antibiotics (Mycoseltm)
Brain Heart Infusion Agar
Potato Dextrose Agar
Cornmeal Agar
Cream of Rice Agar with Tween 80
Germ Tube Media
Rice Grains

Section II. Reagents
Lactophenol Cotton Blue Mounting Media
Potassium Hydroxide Solutions
Periodic Acid-Schiff Stain Reagents
Exercises
Solutions


Lesson 10 - Maintaining Stock Cultures

Maintenance of 'Working' Stock Cultures
Placing Stock Cultures in Sterile Distilled Water
Placing Stock Cultures Under Oil
Recovering Organisms from Water Stock
Recovering Organisms from Oil Shock
Exercises
Solutions


Lesson 11 - Commercial Kits

API* 20C Identification System
Minitektm Yeast Carbon Assimilation Test
Uni-Yeast-Tektm Yeast Identification System
API* Yeast-Identtm System
Exercises
Solutions
Appendix - Glossary of Terms


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